Characterization of high mountain glacier microbiota following inundation with wildfire soot
Section 1: Overview
Name of Research Project
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Related Project
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GWF-NGS: Next Generation Solutions for Healthy Water Resources
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Program Affiliations
Related Research Project(s)
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GWF-NGS: Next Generation Solutions for Healthy Water Resources | |
Dataset Title
Characterization of high mountain glacier microbiota following inundation with wildfire soot
Additional Information
Creators and Contributors
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Milena Esser | Author | | University of Saskatchewan |
Phillip Ankley | Author | | University of Saskatchewan |
Caroline Aubry-Wake | Author | | University of Saskatchewan |
Yuwei Xie | Author | | University of Saskatchewan |
Helen Baulch | Author | | University of Saskatchewan |
Cameron Hoggarth | Author | | University of Saskatchewan |
Markus Hecker | Author | | University of Saskatchewan |
Henner Hollert | Author | | Goethe University Frankfurt |
John Giesy | Author | | University of Saskatchewan |
John Pomeroy | Author | | University of Saskatchewan |
Markus Brinkmann | Author; Point of Contact | markus.brinkmann@usask.ca | University of Saskatchewan |
Abstract
Glacier ecosystems are shrinking at an accelerating rate due to changes in climate, but also due to increased deposition of soot, which has resulted in darkening of glacier surfaces and subsequent changes in absorption of light, associated heat, and altered microbial communities. In this study, compositions of microbial communities on surfaces of the Athabasca Glacier (Alberta, Canada) were measured by use of DNA metabarcoding. Three matrices, glacier ice, cryoconite hole, and glacial surface sediment, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata.
Purpose
Plain Language Summary
Keywords
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Athabasca Glacier |
ecosystem |
microbiome |
soot |
deposition |
PAHs |
Citations
Esser, M., Ankley, P., Aubry-Wake, C., Xie, Y., Baulch, H., Hoggarth, C., Hecker, M., Hollert, H., Giesy, J., Pomeroy, J., Brinkmann, M. (2022). Characterization of high mountain glacier microbiota following inundation with wildfire soot [Dataset]. Federated Research Data Repository.
https://doi.org/10.20383/102.0528
Section 3: Status and Provenance
Dataset Version
1
Dataset Creation Date
2022-01-01
Status of data collection/production
Dataset Completion or Abandonment Date
2022-01-01
Data Update Frequency
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Section 4: Access and Downloads
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.fastq
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