Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment

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Publication Additional Information Download

Publication Type

Journal Article

Authorship

Lingzi Ma, Zhicheng Huang and Juewen Liu

Title

Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment

Year

2021

Publication Outlet

Chemical Communications, 57, 7641-7644

DOI

https://doi.org/10.1039/D1CC03102C

Citation

Lingzi Ma, Zhicheng Huang and Juewen Liu, Selection of a Self-Cleaving Ribozyme Activated in Chemically and Thermally Denaturing Environment. Chemical Communications, 57, 7641-7644, https://doi.org/10.1039/D1CC03102C , 2021.

Abstract

A new self-cleaving ribozyme was obtained from in vitro selection, displaying site-specific cleavage activity under denaturing conditions, such as high temperatures up to 95 °C, and denaturing solvents (20 M formamide). Adding salt such as Mg2+ also inhibited its activity. The conserved ribozyme sequence was found in the genome of several extremophiles, suggesting its potential biological relevance. This study provides an example of a ribozyme working under exotic conditions, which may expand the application of ribozymes in non-biological environments.

Program Affiliations

GWF: Global Water Futures

Project Affiliations

GWF-SSSWQM: Sensors and Sensing Systems for Water Quality Monitoring

Publication Stage

Published

Additional Information

Sensor and Sensing Systems for Water Quality Monitoring 2, Refereed Publications

Download Links

https://doi.org/10.1039/D1CC03102C