A general transition metal binding aptamer following the Irving–Williams series

This dataset is cited by https://pubs.rsc.org/en/content/articlepdf/2025/SC/D5SC02436F?page=search

Using both Co2+ and Ni2+ as target metal ions, aptamer selections were carried out by
immobilization of a DNA library via hybridization. A set of aptamers were obtained although they
all showed binding to both metal ions. The Co-1 aptamer was highly enriched in both selections
and it is a general metal binding aptamer with binding affinity following the Irving-Williams series:
Mn2+ < Fe2+ < Co2+ < Ni2+ < Cu2+ > Zn2+. Using NMR spectroscopy, Co-1 forms new base pairs with a relatively large structural change upon binding to Zn2+, whereas a previously reported Zn1 aptamer has little conformational change upon Zn2+ binding. The induced-fitting of the Co-1
aptamer and the lock-and-key binding of Zn-1 aptamer may explain the general metal binding of
the former. Using isothermal titration calorimetry, Co-1 binding Co2+ was an enthalpy-driven
event and the Kd was determined to be 2.7 µM, whereas the Kd from the strand-displacement assay
was 76 nM. The Co-1 aptamer is the first reported aptamer to follow the Irving-Williams series,
although this series is very prevalent in proteins. This aptamer can thus serve as a model system
for understanding metal binding by DNA and can also be a general first-row transition metal
sensing element.

Wang, J., Liu, J. (2025). A general transition metal binding aptamer following the Irving–Williams series. Federated Research Data Repository. https://doi.org/10.20383/103.01328

University of Waterloo, Waterloo, Ontario, Canada and South China Agricultural University, Guangzhou, Guangdong, China

These data are available under a CC BY 4.0 license < https://creativecommons.org/licenses/by/4.0/ >

https://doi.org/10.20383/103.01328